High-occurrence hereditary map construction when you look at the maize full-sib families
Having training intraspecific type away from recombination price inside the maize, i composed a couple half of-sib boards, that getting Dent plus one to own Flint inbred outlines. Drop and Flint are a couple of maize gene swimming pools which can be very important for crossbreed reproduction inside the European countries . The 2 50 % of-sib boards comprised all in all, 24 complete-sib group. The brand new Reduction panel comprises ten crosses off a main Damage inbred line (F353) which have diverse Damage inventor inbreds of the Eu Dent gene pond which were created in European countries and North america. About Flint panel a central Flint inbred range (UH007) is actually entered in order to eleven genetically diverse Flint maker inbreds of one’s European Flint reproduction procedure. The fresh Flint founder lines result from early maize introductions to Spain and off so-titled North Flints from France and you can Germany. At the same time, one or two mutual crosses between the two central Damage and you will Flint traces was basically analyzed plus for every single committee you to cross to your United states Dent line B73 is integrated as the a link with the us nested organization mapping (NAM) society .
Here, we report on the analysis of the variation of recombination rate in 23 populations of maize using SNPs from the MaizeSNP50 BeadChip . All populations consist of doubled haploid (DH) lines obtained by in vivo haploid induction . DH lines produced with this method reflect female meiosis. Unlike F2 or RILs, DH lines have the great advantage that the genetic information of each gamete is directly observed. Our vietnamcupid recenze objectives were to analyze intraspecific variation for (1) GWRR and chromosome-wide recombination rate, (2) recombination landscape along chromosomes, and (3) CO interference, using high-density genetic linkage maps. We found significant differences of GWRR between individual populations as well as between Dent ? Dent versus Flint ? Flint populations, but not between the Dent and Flint gene pools in general. GWRR was not correlated with the genetic structure of the lines as inferred from admixture analysis. We analyzed the recombination landscapes in all populations and found significant differences between individual populations and between pools. Finally, we characterized quantitatively the interfering and non-interfering CO formation pathways and found a negative correlation over all chromosomes between interference intensity of pathway P1 and GWRR.
We constructed two half-sib panels comprising a total of 24 maize full-sib families of DH lines for the analysis of intraspecific variation of recombination rates. The full-sib families within the two panels represent the diversity of important founder lines of the European Dent and Flint germplasm, respectively (Table 1; Additional file 1). In the Dent panel (prefix CFD), all full-sib families have the same common parental line F353 which was crossed to diverse Dent founder lines. The common parent of the Flint full-sib families (prefix CFF) is line UH007, which was crossed to diverse Flint founder lines. 1 plants for in vivo haploid induction, so our maps reflect only female meioses. Details on the diversity analysis of the parental lines are given in Additional file 2.
In most crosses, the fresh haploid inducer range was used once the men moms and dad when entered with the F
Because an initial part of the analysis out-of recombination, personal hereditary charts have been built for 23 out of the 24 communities and each of the ten chromosomes. Map statistics are offered for the Desk step 1 with info within the Most document step three, plus the done a number of marker ranking in every maps with new raw segregation information is given into the More document 4. As a whole, 39,439 SNPs of one’s MaizeSNP50 range would-be mapped inside the from the minimum one population. Over-all populations, doing 33,884 COs were observed in 2,233 female meioses and you can, on average, 11,988 SNPs were mapped in a single given inhabitants. The common having Reduction ? Drop communities (all the CFD but CFD01) is actually 13,247 markers mapped as well as for Flint ? Flint populations (all CFF except CFF01 and CFF02) the common is nine,874. To possess society CFF05, hence made-up just 34 DHs, no stable chart might possibly be received. The complete length of hereditary maps ranged from a single,180 centiMorgan (cM) inside population CFD05 to just one,893 cM for the CFF03 with a suggest of 1,508 ± 185 cM (suggest ± standard departure). An average hereditary map duration in the ten Damage ? Reduction communities (CFD02 to CFD12) was 1,353 ± 99 cM, given that 11 hereditary maps regarding Flint ? Flint populations (CFF03 to help you CFF15) were, an average of, longer in the 1,645 ± 154 cM. The 3 maps regarding crosses between Dent and you may Flint moms and dads (CFD01, CFF01, CFF02) had an intermediate average amount of step one,570 ± 85 cM. The largest gap into the an inherited map is actually 53.seven cM on long-arm away from chromosome 7 (7L) within the population CFF13, in which zero SNPs were polymorphic within parents, most likely due to identity-by-origin (IBD). Facts about genome-wider variety and you can IBD areas ranging from mothers of the mapping communities have been in Additional file 2. Synteny and you can colinearity of one’s genetic maps try in contrast to the fresh B73 AGPv2 genome set up. As a whole, a high contract try receive, indicating the fresh higher structure regarding marker purchase across populations, with pair exclusions while the described inside the More file dos.